《植物生理学报》 2016, 52(4): 461-470

铝胁迫对水稻膜脂组分和含量的影响

王鑫月^1,2, 张梅娟³, 熊炳霖^1,2, 殷俐娜^1,2,3,*, 王仕稳^1,2,3, 邓西平^1,2,3

¹中国科学院水利部水土保持研究所, 黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西杨陵712100; ²中国科学院大学, 北京100049; ³西北农林科技大学水土保持研究所, 陕西杨陵712100

通信作者：殷俐娜；E-mail: linayin@nwsuaf.edu.cn

摘　要：

以‘日本晴’水稻为试验材料, 研究铝胁迫对水稻幼苗生长的影响, 及铝胁迫下其根系和功能叶片中脂质含量及组分的变化。结果表明Al处理显著抑制了水稻根尖的伸长和生长, 降低了植株的生物量以及叶绿素含量。铝胁迫后, 半乳糖脂含量变化显著, 根和叶中的单半乳糖甘油二酯(MGDG)和双半乳糖甘油二酯(DGDG)含量均降低; 而磷脂(PL)及硫代异鼠李糖甘油二酯(SQDG)的含量在根中表现为上升, 在叶中则显著降低。此外, 根和叶中的脂肪酸不饱和度均降低。由此可见, 铝胁迫引起水稻膜脂组分和含量的变化, 这种变化可能是铝抑制植物生长的重要原因之一。

关键词：水稻; 铝胁迫; 细胞膜; 膜脂

收稿：2015-11-18   修定：2016-03-31

资助：国家自然科学基金(31200206)和中国科学院西部之光项目。

Effects of aluminum stress on the composition and content of membrane lipids in rice (Oryza sativa)

WANG Xin-Yue^1,2, ZHANG Mei-Juan³, XIONG Bing-Lin^1,2, YIN Li-Na^1,2,3,*, WANG Shi-Wen^1,2,3, DENG Xi-Ping^1,2,3

¹State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi 712100, China; ²University of Chinese Academy of Science, Beijing 100049, China; ³Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi 712100, China

Corresponding author: YIN Li-Na; E-mail: linayin@nwsuaf.edu.cn

Abstract:

The presence of aluminum (Al) in acidic soils is a major abiotic stress limiting the production of crops. The objective of this study was to evaluate the effects of Al on membrane lipid composition and content in the roots and shoots of rice (Oryza sativa L. cv. Nipponbare) plants grown under hydroponic conditions. After exposed to 100 μmol•L^-1 Al (pH 4.5) for 48 hours, the growth of root tips was inhibited and the biomass of shoots and roots was decreased significantly. Meanwhile, Al treatment also caused a decrease in chlorophyll content. The lipid analysis showed that the levels of galactolipids, including monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) were decreased under Al treatment in both roots and leaves. Additionally, the contents of phospholipids (PL) and sulfoquinovosyldiacylglycerol (SQDG) were also changed under Al stress. Al also caused a decrease in membrane fluidity since the double bond index in galactolipids and phospholipids were decreased. Taken together, the lipid content and composition were changed significantly under Al stress in rice, and this change may lead to the decrease in membrane fluidity and increase in membrane permeability, which lead to damages in membrane structure and function, and finally inhibit plant growth.

Key words: rice (Oryza sativa L. cv. Nipponbare); Al stress; cell membrane; membrane lipid